Sensitive Immunosensor Developed for Accurate Quantification of Alpha-Fetoprotein via Evanescent Field-Enhanced Fluorescence Imaging

By:Admin on 2023-05-09 02:42:51

Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Alpha-fetoprotein (AFP) is a protein that is mainly produced during pregnancy in the developing fetus. However, in some cases, elevated levels of AFP in adults can indicate a potential risk for developing liver cancer. In order to accurately and effectively detect the levels of AFP in adult blood samples, researchers have developed a highly sensitive immunosensor based on a gold nanopatterned chip. This biosensor works by creating a sandwich of antigen between the AFP antibody on the chip, which has been modified with protein A/G, and the secondary antibody, which is a monoclonal anti-human-AFP labeled with biotin (biotin-labeled anti-AFP). The AFP concentration is then determined based on evanescent field fluorescence signal, which is generated by the interaction between biotin-labeled anti-AFP and a streptavidin-labeled fluorescence dye. The gold nanopatterned chip is specially modified with dithiobis(succinimidyl propionate) and protein A/G, which allows for the immobilization of the AFP antibody. This technology enhances the sensitivity of the immunoassay, as it allows for the AFP concentration to be measured in a wide dynamic linear range of 720 zM–10 nM, with a detection limit of 720 zM. This technology has significant benefits over conventional chemiluminescence immunosensors, as it achieves a much higher sensitivity of about 40,000-fold. This makes it a valuable tool for clinical assays of AFP. The immunoassay can detect a wide range of AFP concentrations with high accuracy and sensitivity. One of the key features of this technology is the use of biotin-based immunoassays. Biotin is a water-soluble vitamin that plays a vital role in various metabolic functions in the human body. Because of its high binding affinity to streptavidin, biotin can be used as a label for a variety of immunoassays. This technology utilizes biotin-labeled anti-AFP antibody on the chip, which enhances the sensitivity of the immunoassay. In conclusion, the single-molecule sandwich immunoassay based on a gold nanopatterned chip is a major breakthrough in the accurate and effective detection of AFP in clinical assays. The use of biotin-based immunoassays enhances the sensitivity of the immunoassay, which makes it a valuable tool for early detection of liver cancer in patients with elevated levels of AFP. The potential applications of this technology could be limitless, and it could pave the way for future developments in immunosensing.

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Effective Vitamin B12 ELISA Kit for Human Detection with High Sensitivity and Wide Range of Reactivity

By:Admin on 2023-05-09 02:41:58

Vitamin B12 is an essential nutrient that is required by our body for various functions, including the production of red blood cells and maintenance of the central nervous system. It is primarily found in animal-based foods such as meat, fish, and dairy products. However, in some cases, people may have a deficiency of this vital nutrient due to various reasons, including inadequate intake, certain medical conditions, and lifestyle choices. To detect the levels of vitamin B12 in the human body, various methods are available, including blood tests, imaging tests, and biopsies. Of these, the most common method is the use of ELISA kits, which are highly sensitive, specific, and reliable. One such ELISA kit is the Human Vitamin B12 ELISA Kit, manufactured by Biorbyt. This kit is designed to detect vitamin B12 in human serum and plasma samples. The kit is specific to human vitamin B12 and does not cross-react with other forms of vitamin B12 or related compounds. The Human Vitamin B12 ELISA Kit has a range of 1.56 pg/mL to 100 pg/mL, which can detect even the lowest levels of vitamin B12 in the human body. The sensitivity of the kit is 1.498 pg/mL, which means it can detect even small changes in vitamin B12 levels. The kit is easy to use and requires only small sample volumes (50-100 μL). The detection wavelength is 450 nm, and the assay time is approximately 3 hours. The Human Vitamin B12 ELISA Kit has been tested for ELISA applications and has shown good accuracy and precision. The kit can be used in various research and diagnostic applications, including the diagnosis and monitoring of vitamin B12 deficiency. Vitamin B12 deficiency can cause anemia, fatigue, weakness, and other symptoms, and it is prevalent among older adults, vegetarians and vegans, and people with certain medical conditions such as Crohn's disease and celiac disease. In conclusion, the Human Vitamin B12 ELISA Kit is an essential tool for researchers and clinicians to detect vitamin B12 levels accurately and efficiently. The kit is highly sensitive, specific, and reliable, and it can be used in various research and diagnostic applications. If you are looking for a reliable and accurate Vitamin B12 Elisa Kit, the Human Vitamin B12 ELISA Kit manufactured by Biorbyt is an excellent choice.

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Latest Developments in ELISA Testing: News, Videos, and Photos of ELISA Test Kits. Stay Up-to-Date with ELISA Testing News and Updates.

By:Admin on 2023-05-09 02:41:15

The Elisa Test Kit: A Vital Tool in Modern MedicineIn the world of modern medicine, it is critical to have tools that can accurately and quickly diagnose diseases and infections. One such tool that has revolutionized the medical industry is the Elisa Test Kit.The Elisa Test Kit, short for Enzyme-Linked Immunosorbent Assay Test Kit, is a diagnostic tool that uses antibodies and color change to detect the presence of a particular disease or infection.How does it work?The Elisa Test Kit works on the basis of the immune system’s response to a particular disease or infection. When a foreign substance enters the body, the immune system produces antibodies to fight against it.The Elisa Test Kit contains a substrate that consists of a specific antigen that is associated with the disease or infection. When the patient's blood sample is added to the substrate, any antibodies that are present in the blood will bind to the antigen.The Elisa Test Kit then uses an Enzyme-Linked secondary antibody to attach another specific enzyme to the bound complex. The presence of the enzyme is then detected using a color change reaction.Advantages of the Elisa Test Kit:There are many advantages to using the Elisa Test Kit as a diagnostic tool. Some of these include:1. High Accuracy: The Elisa Test Kit is known for its high accuracy in detecting specific diseases or infections, making it a reliable tool for physicians.2. Quick Results: The Elisa Test Kit provides fast results, allowing physicians to diagnose diseases or infections quickly and take necessary actions.3. Versatility: The Elisa Test Kit can be used to detect a wide range of diseases and infections, making it a versatile tool in modern medicine.Applications of the Elisa Test Kit:The Elisa Test Kit has been widely used in many fields, including clinical diagnosis, research, and quality control. Some of its applications include:1. Identifying Viral Infections: The Elisa Test Kit is commonly used to identify viral infections such as HIV, Hepatitis B and C, and Zika virus.2. Cancer Detection: The Elisa Test Kit can be used to detect cancer markers, allowing physicians to diagnose and monitor cancer patients.3. Food Allergy Testing: The Elisa Test Kit can also be used to identify food allergies, allowing individuals to avoid certain foods that may cause allergic reactions.In conclusion, the Elisa Test Kit is a vital tool in modern medicine that has revolutionized the way diseases and infections are diagnosed. Its high accuracy and fast results have made it a reliable diagnostic tool for physicians, and its versatility has made it an essential tool in many fields of medical research.

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